Review




Structured Review

InterPro Inc xklp2 targeting protein (interpro:ipr009675)
Xklp2 Targeting Protein (Interpro:Ipr009675), supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/targeting+for+xklp2/targeting+for+xklp2/pmc06481908__plz019_suppl_supplementary_tables-77-30-31
Average 90 stars, based on 1 article reviews
xklp2 targeting protein (interpro:ipr009675) - by Bioz Stars, 2026-10
90/100 stars

Images

Related Articles

other:

Article Title: mRNA-seq Analysis of the Gossypium arboreum transcriptome Reveals Tissue Selective Signaling in Response to Water Stress during Seedling Stage
Article Snippet: Unigene5348_All//0.00E+00//TC150772 nr + Unigene53488_All 242 0.00 2.72 1.10 0.21 0.57 1.31 0.00 0.00 0.00 1.14E+01 6.25E-04 Up 1.01E+01 8.00E-02 1.47E+00 6.18E-01 2.67E+00 1.44E-01 null null null null Unigene5349_All 735 87.20 114.51 124.90 51.44 34.39 33.61 11.68 28.03 2.11 3.93E-01 3.29E-12 5.18E-01 9.19E-21 -5.81E-01 2.32E-13 -6.14E-01 3.36E-13 1.26E+00 4.99E-27 Up -2.47E+00 2.73E-29 Down Unigene5349_All//gi|224128011|ref| XP_002320219.1|//2.00E-47//SAUR family protein [Populus trichocarpa] >gi|222860992|gb|EEE98534.1| SAUR family protein [Populus trichocarpa] Unigene5349_All//5.00E33//AT2G46690| auxin-responsive family protein Unigene5349_All//0.00E+00//TC174243 nr + Unigene53495_All 289 0.00 0.00 0.00 0.34 0.00 0.31 74.17 51.05 166.38 null null null null -8.43E+00 3.12E-01 -1.42E-01 9.49E-01 -5.39E-01 3.23E-07 1.17E+00 6.32E-56 Up Unigene53495_All//gi|40218371|gb|A AR83118.1|//3.00E-25//secretory laccase [Gossypium arboreum] Unigene53495_All//2.00E07//AT5G48100| TT10 (TRANSPARENT TESTA 10); copper ion binding / laccase Unigene53495_All//8.00E-76//TC137114 GO:0005576//GO:0009808//GO:0016682 //GO:0046914 nr + Unigene53497_All 514 0.00 0.00 0.00 0.00 0.00 0.00 46.23 15.69 9.69 null null null null null null null null -1.56E+00 1.95E-42 Down -2.25E+00 1.22E-71 Down Unigene53497_All//gi|30725286|gb|A AP37665.1|//1.00E-36//At2g21045 [Arabidopsis thaliana] Unigene53497_All//6.00E38//AT2G21045| INVOLVED IN: aging; EXPRESSED IN: hypocotyl, root; CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G66170.2); Has 1919 Blast hits to 1917 proteins in 542 species: Archae - 34; Bacteria - 1258; Metazoa - 17; Fungi - 21; Plants - 115; Viruses - 0; Other Eukaryotes - 474 (source: NCBI BLink).

Article Title: mRNA-seq Analysis of the Gossypium arboreum transcriptome Reveals Tissue Selective Signaling in Response to Water Stress during Seedling Stage
Article Snippet: Unigene11935_All 3392 0.69 0.60 0.68 4.08 1.50 1.64 8.56 22.64 6.84 -2.16E-01 5.99E-01 -4.03E-02 9.38E-01 -1.44E+00 1.82E-22 Down -1.32E+00 2.69E-17 Down 1.40E+00 1.23E-115 Up -3.25E-01 2.18E-04 Unigene11935_All//gi|223452528|gb| ACM89591.1|//0.00E+00//leucinerich repeat family protein / protein kinase family protein [Glycine max] Unigene11935_All//8.00E172//AT4G08850| kinase Unigene11935_All//0.00E+00//gi|3326428|gb |AI055314.1|AI055314 ko04626|Plant-pathogen interaction GO:0000166//GO:0004672//GO:0044464 nr + Unigene119353_All 333 0.00 0.00 0.80 0.30 0.52 0.14 1.52 1.63 5.14 null null 9.64E+00 8.03E-02 7.89E-01 7.71E-01 -1.14E+00 6.77E-01 9.89E-02 9.21E-01 1.76E+00 1.93E-04 Up Unigene119353_All//gi|116317776|em b|CAH65754.1|//6.00E06//OSIGBa0123D13.3 [Oryza sativa (indica cultivar-group)] Unigene119353_All//1.00E05//AT5G64460| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Phosphoglycerate mutase (InterPro:IPR013078); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G58280.3); Has 385 Blast hits to 382 proteins in 84 species: Archae - 0; Bacteria - 19; Metazoa - 2; Fungi - 201; Plants - 67; Viruses - 0; Other Eukaryotes - 96 (source: NCBI BLink).

Article Title: mRNA-seq Analysis of the Gossypium arboreum transcriptome Reveals Tissue Selective Signaling in Response to Water Stress during Seedling Stage
Article Snippet: Unigene33498_All//3.00E-114//TC157812 nr + Unigene3350_All 1120 4.30 1.90 3.00 7.52 7.07 5.00 12.27 5.34 4.94 -1.18E+00 2.86E-05 Down -5.21E-01 7.30E-02 -8.81E-02 7.20E-01 -5.88E-01 2.79E-03 -1.20E+00 5.57E-17 Down -1.31E+00 5.71E-20 Down Unigene3350_All//gi|16323093|gb|AA L15281.1|//2.00E42//At1g73940/F2P9_19 [Arabidopsis thaliana] >gi|21360419|gb|AAM47325.1| At1g73940/F2P9_19 [Arabidopsis thaliana] Unigene3350_All//3.00E40//AT1G73940| unknown protein Unigene3350_All//0.00E+00//TC149823 GO:0043231 nr - Unigene33502_All 361 4.49 1.97 3.69 4.00 2.48 9.51 4.22 9.38 2.10 -1.19E+00 2.32E-02 -2.85E-01 6.21E-01 -6.90E-01 2.06E-01 1.25E+00 2.28E-04 Up 1.15E+00 1.58E-04 Up -1.01E+00 2.94E-02 Unigene33502_All//gi|116309951|emb |CAH66982.1|//3.00E-40//H0714H04.9 [Oryza sativa (indica cultivargroup)] Unigene33502_All//1.00E33//AT1G48920| ATNUC-L1; nucleic acid binding / nucleotide binding Unigene33502_All//2.00E151//gi|13351841|gb|BG442189.1|BG442189 nr + Unigene33505_All 2379 28.85 10.44 11.68 49.06 47.94 63.75 126.68 67.89 92.92 -1.47E+00 9.24E-91 Down -1.30E+00 1.98E-73 Down -3.33E-02 5.91E-01 3.78E-01 2.94E-21 -9.00E-01 3.32E-222 -4.47E-01 2.28E-66 Unigene33505_All//gi|21700195|dbj| BAC02896.1|//1.00E-162//tobacco nucleolin [Nicotiana tabacum] Unigene33505_All//7.00E53//AT1G48920| ATNUC-L1; nucleic acid binding / nucleotide binding Unigene33505_All//0.00E+00//gi|164250348| gb|ES814502.1|ES814502 nr - Unigene33506_All 1109 2.97 2.10 2.59 4.04 3.60 5.29 6.10 1.88 5.07 -5.00E-01 1.44E-01 -1.97E-01 6.17E-01 -1.67E-01 6.06E-01 3.89E-01 1.31E-01 -1.69E+00 3.80E-14 Down -2.68E-01 1.95E-01 Unigene33506_All//gi|49533766|gb|A AT66765.1|//1.00E-18//Putative selenium-binding protein, related [Solanum demissum] Unigene33506_All//1.00E10//AT2G25580| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 21 plant structures; EXPRESSED DURING: 12 growth stages; CONTAINS InterPro DOMAIN/s: Pentatricopeptide repeat (InterPro:IPR002885); BEST Arabidopsis thaliana protein match is: pentatricopeptide (PPR) repeat-containing protein (TAIR:AT4G32450.1); Has 7881 Blast hits to 3723 proteins in 97 species: Archae - 0; Bacteria - 6; Metazoa - 6; Fungi - 16; Plants - 7829; Viruses - 0; Other Eukaryotes - 24 (source: NCBI BLink).

Article Title: mRNA-seq Analysis of the Gossypium arboreum transcriptome Reveals Tissue Selective Signaling in Response to Water Stress during Seedling Stage
Article Snippet: nr + Unigene4085_All 1628 2.86 2.02 1.80 7.56 6.58 4.19 2.99 1.49 2.69 -5.00E-01 7.65E-02 -6.69E-01 2.23E-02 -2.00E-01 2.58E-01 -8.51E-01 6.98E-08 -1.00E+00 4.32E-05 Down -1.50E-01 5.82E-01 Unigene4085_All//gi|4914316|gb|AAD 32864.1|AC005489_2//7.00E15//F14N23.2 [Arabidopsis thaliana] >gi|107738163|gb|ABF83653.1| At1g10140 [Arabidopsis thaliana] Unigene4085_All//3.00E16//AT1G10140| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Uncharacterised conserved protein UCP031279 (InterPro:IPR016972); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G58420.1); Has 38 Blast hits to 38 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 33; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink).

Bacteria:

Article Title: mRNA-seq Analysis of the Gossypium arboreum transcriptome Reveals Tissue Selective Signaling in Response to Water Stress during Seedling Stage
Article Snippet: Unigene1391_All//0.00E+00//gi|78353890|gb |DT574164.1|DT574164 nr - Unigene13910_All 1609 6.41 5.64 3.28 19.20 21.70 14.74 16.08 3.74 5.65 -1.85E-01 3.48E-01 -9.67E-01 2.33E-07 1.77E-01 6.41E-02 -3.81E-01 5.92E-05 -2.10E+00 2.33E-69 Down -1.51E+00 2.01E-45 Down Unigene13910_All//gi|15218562|ref| NP_177411.1|//1.00E-77//iqd8 (IQdomain 8); calmodulin binding [Arabidopsis thaliana] >gi|38016019|gb|AAR07516.1| At1g72670 [Arabidopsis thaliana] Unigene13910_All//4.00E66//AT1G72670| iqd8 (IQ-domain 8); calmodulin binding Unigene13910_All//0.00E+00//gi|78335607|g b|DT555881.1|DT555881 nr + Unigene13916_All 895 12.86 14.89 13.86 29.05 34.05 32.30 39.78 32.36 19.59 2.11E-01 2.04E-01 1.08E-01 5.77E-01 2.29E-01 2.04E-02 1.53E-01 1.83E-01 -2.98E-01 2.39E-04 -1.02E+00 4.46E-34 Down Unigene13916_All//gi|18404934|ref| NP_565899.1|//5.00E29//hydroxyproline-rich glycoprotein family protein [Arabidopsis thaliana] >gi|15724197|gb|AAL06490.1|AF41180 1_1 At2g39050/T7F6.22 [Arabidopsis thaliana] >gi|20147403|gb|AAM10411.1| At2g39050/T7F6.22 [Arabidopsis thaliana] >gi|20197446|gb|AAC79615.2| expressed protein [Arabidopsis thaliana] Unigene13916_All//2.00E30//AT2G39050| hydroxyproline-rich glycoprotein family protein Unigene13916_All//0.00E+00//gi|84165402|g b|DR454050.1|DR454050 nr + Unigene1392_All 878 0.54 0.35 0.30 1.70 2.31 1.34 2.41 3.05 4.90 -6.31E-01 5.54E-01 -8.23E-01 4.57E-01 4.43E-01 3.67E-01 -3.48E-01 5.60E-01 3.40E-01 3.69E-01 1.03E+00 7.08E-05 Up Unigene1392_All//gi|30387531|gb|AA P31931.1|//5.00E-58//At3g26670 [Arabidopsis thaliana] Unigene1392_All//2.00E59//AT3G26670| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF803 (InterPro:IPR008521); BEST Arabidopsis thaliana protein match is: permease-related (TAIR:AT3G23870.1); Has 715 Blast hits to 711 proteins in 119 species: Archae - 0; Bacteria - 0; Metazoa - 298; Fungi - 244; Plants - 119; Viruses - 2; Other Eukaryotes - 52 (source: NCBI BLink). .. Unigene14480_All 942 5.22 4.73 6.05 10.95 10.09 7.96 3.37 1.60 1.86 -1.41E-01 6.17E-01 2.12E-01 4.34E-01 -1.18E-01 5.83E-01 -4.60E-01 9.77E-03 -1.08E+00 6.26E-04 Down -8.55E-01 4.42E-03 Unigene14480_All//gi|297811075|ref |XP_002873421.1|//2.00E72//ribosomal protein L17 family protein [Arabidopsis lyrata subsp. lyrata] >gi|297319258|gb|EFH49680.1| ribosomal protein L17 family protein [Arabidopsis lyrata subsp. lyrata] Unigene14480_All//1.00E56//AT5G64650| ribosomal protein L17 family protein Unigene14480_All//0.00E+00//TC130223 ko03010|Ribosome GO:0005198//GO:0010467//GO:0030529 //GO:0043231 nr - Unigene14481_All 2055 1.32 3.80 2.23 2.01 6.10 5.27 9.18 8.16 5.37 1.52E+00 1.96E-11 Up 7.51E-01 1.06E-02 1.60E+00 3.36E-22 Up 1.39E+00 1.52E-14 Up -1.70E-01 1.78E-01 -7.73E-01 7.43E-12 Unigene14481_All//gi|5902406|gb|AA D55508.1|AC008148_18//1.00E31//Hypothetical protein [Arabidopsis thaliana] >gi|46518435|gb|AAS99699.1| At1g70950 [Arabidopsis thaliana] Unigene14481_All//2.00E32//AT1G70950| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: 4 anthesis, petal differentiation and expansion stage, D bilateral stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Targeting for Xklp2 (InterPro:IPR009675); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G23060.1); Has 875 Blast hits to 771 proteins in 143 species: Archae - 0; Bacteria - 102; Metazoa - 281; Fungi - 65; Plants - 205; Viruses - 3; Other Eukaryotes - 219 (source: NCBI BLink). .. Unigene13925_All 967 3.79 1.94 3.69 8.96 9.40 4.06 2.40 1.12 1.44 -9.66E-01 2.76E-03 -3.78E-02 9.21E-01 6.88E-02 8.01E-01 -1.14E+00 6.24E-09 Down -1.10E+00 3.58E-03 -7.38E-01 3.95E-02 Unigene13925_All//gi|115445171|ref |NP_001046365.1|//7.00E58//Os02g0230200 [Oryza sativa Japonica Group] >gi|113535896|dbj|BAF08279.1| Os02g0230200 [Oryza sativa Japonica Group] Unigene13925_All//7.00E87//AT3G58800| unknown protein Unigene13925_All//0.00E+00//ES828671 nr + Unigene13929_All 1489 8.85 2.52 7.44 8.70 10.31 10.62 4.46 8.53 4.60 -1.81E+00 3.87E-24 Down -2.51E-01 1.42E-01 2.46E-01 1.01E-01 2.88E-01 5.02E-02 9.37E-01 9.00E-11 4.58E-02 8.35E-01 Unigene13929_All//gi|18411876|ref| NP_567110.1|//3.00E-58//zinc finger (C3HC4-type RING finger) family protein [Arabidopsis thaliana] Unigene13929_All//2.00E91//AT3G61180| zinc finger (C3HC4type RING finger) family protein Unigene13929_All//0.00E+00//gi|78344708|g b|DT564982.1|DT564982 nr + Unigene1393_All 1539 3.77 7.61 2.87 8.67 9.35 6.69 22.69 41.23 44.91 1.01E+00 3.02E-09 Up -3.94E-01 1.41E-01 1.09E-01 5.46E-01 -3.75E-01 1.63E-02 8.61E-01 5.70E-44 9.85E-01 4.57E-61 Unigene1393_All//gi|30387531|gb|AA P31931.1|//1.00E-171//At3g26670 [Arabidopsis thaliana] Unigene1393_All//2.00E163//AT3G26670| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF803 (InterPro:IPR008521); BEST Arabidopsis thaliana protein match is: permease-related (TAIR:AT3G23870.1); Has 715 Blast hits to 711 proteins in 119 species: Archae - 0; Bacteria - 0; Metazoa - 298; Fungi - 244; Plants - 119; Viruses - 2; Other Eukaryotes - 52 (source: NCBI BLink).

Article Title: mRNA-seq Analysis of the Gossypium arboreum transcriptome Reveals Tissue Selective Signaling in Response to Water Stress during Seedling Stage
Article Snippet: .. Unigene33765_All 1847 134.53 95.41 130.43 135.23 148.22 100.43 44.16 21.14 26.10 -4.96E-01 1.10E-52 -4.46E-02 2.51E-01 1.32E-01 4.54E-06 -4.29E-01 5.38E-44 -1.06E+00 1.02E-79 Down -7.59E-01 9.66E-47 Unigene33765_All//gi|226533114|ref |NP_001148491.1|//1.00E58//LOC100282106 [Zea mays] >gi|195619766|gb|ACG31713.1| seed specific protein Bn15D14A [Zea mays] Unigene33765_All//3.00E75//AT4G32330| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Targeting for Xklp2 (InterPro:IPR009675); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G25480.1); Has 4728 Blast hits to 3313 proteins in 331 species: Archae - 4; Bacteria - 347; Metazoa - 2216; Fungi - 461; Plants - 233; Viruses - 22; Other Eukaryotes - 1445 (source: NCBI BLink). .. Unigene28991_All 845 0.37 0.60 1.26 1.24 1.02 1.55 1.95 0.30 1.23 6.91E-01 4.99E-01 1.76E+00 1.85E-02 -2.81E-01 6.74E-01 3.24E-01 6.37E-01 -2.72E+00 1.39E-06 Down -6.71E-01 1.27E-01 Unigene28991_All//gi|124359343|gb| ABD28505.2|//7.00E-43//RNAdirected DNA polymerase (Reverse transcriptase); Polynucleotidyl transferase, Ribonuclease H fold [Medicago truncatula] Unigene28991_All//2.00E18//AT3G24255| FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; CONTAINS InterPro DOMAIN/s: RNAdirected DNA polymerase (reverse transcriptase), related (InterPro:IPR015706); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G23910.1); Has 2302 Blast hits to 2235 proteins in 74 species: Archae - 4; Bacteria - 15; Metazoa - 871; Fungi - 12; Plants - 1295; Viruses - 0; Other Eukaryotes - 105 (source: NCBI BLink).



Similar Products

93
Proteintech targeting protein for xklp2 tpx2 antibodies
Quantitative PCR primers.
Targeting Protein For Xklp2 Tpx2 Antibodies, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/targeting+for+xklp2/TPX2+Antibody/pmc11190816-88-51-64
Average 93 stars, based on 1 article reviews
targeting protein for xklp2 tpx2 antibodies - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

90
InterPro Inc xklp2 targeting protein (interpro:ipr009675)
Quantitative PCR primers.
Xklp2 Targeting Protein (Interpro:Ipr009675), supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/targeting+for+xklp2/targeting+for+xklp2/pmc06481908__plz019_suppl_supplementary_tables-77-30-31
Average 90 stars, based on 1 article reviews
xklp2 targeting protein (interpro:ipr009675) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Novus Biologicals antibodies to tpx2 (targeting protein for xklp2)
Quantitative PCR primers.
Antibodies To Tpx2 (Targeting Protein For Xklp2), supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/targeting+for+xklp2/anti+tpx2/pmc04069685-46-0-12
Average 90 stars, based on 1 article reviews
antibodies to tpx2 (targeting protein for xklp2) - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
InterPro Inc targeting for xklp2
Quantitative PCR primers.
Targeting For Xklp2, supplied by InterPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/targeting+for+xklp2/targeting+for+xklp2/pmc03557298__pone__0054762__s005-56-67-68
Average 90 stars, based on 1 article reviews
targeting for xklp2 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Quantitative PCR primers.

Journal: Oncology Letters

Article Title: Reversal of the tamoxifen‑resistant breast cancer malignant phenotype by proliferation inhibition with bromosulfonamidine amino‑podophyllotoxin

doi: 10.3892/ol.2024.14506

Figure Lengend Snippet: Quantitative PCR primers.

Article Snippet: Mouse anti-human Caspase-9 antibodies (cat. no. 9508S; 1:1,000) were purchased from Cell Signaling Technology, Inc., rabbit anti-human Bcl-2 (cat. no. BA0412; 1:1,000) and cyclin B1 (CCNB1; cat. no. BA0766; 1:1,000) antibodies were purchased from Wuhan Boster Biological Technology, Ltd., rabbit anti-human polo like kinase (PLK)-1 antibodies (cat. no. 10305-1-AP; 1:1,000) and targeting protein for Xklp2 (TPX2) antibodies (cat. no. 11741-1-AP; 1:2,000) were purchased from Proteintech Group, Inc., and the rabbit anti-human PLK-4 antibodies (cat. no. A9863; 1:1,000) were purchased from ABclonal Biotech Co., Ltd.

Techniques: Real-time Polymerase Chain Reaction, Sequencing

Effect of BSAPPT on apoptosis and cycle-related gene or protein expression in MCF-7, MCF7/TAMR and other cancer cells. (A) mRNA expression levels of genes linked to the cell cycle and apoptosis were measured by qPCR both before and after MCF-7 and MCF7/TAMR cells were treated with 10 µg/ml BSAPPT. (B) Western blotting detection of apoptotic and cycle-related protein expression variations in MCF-7 and MCF7/TAMR cells before and after using 10 µg/ml BSAPPT. Results of qPCR analysis that assessed differences in the level of expression of genes linked to the cell cycle and apoptosis before and after (C) A549 and (D) MDA-MB-231 cells were treated with 10 µg/ml BSAPPT. *P<0.05; **P<0.01; ***P<0.001. BSAPPT, bromosulfonamidine amino-podophyllotoxin; qPCR, quantitative PCR; Bcl-2, B-cell lymphoma 2; Caspase, cysteine aspartic acid-specific protease; PLK, polo like kinase; CCNB1, cyclin B1; TPX2, targeting protein for Xklp2; Bax, Bcl-2 associated X; Cyt-C, cytochrome c; Apaf-1, apoptotic protease activating factor 1.

Journal: Oncology Letters

Article Title: Reversal of the tamoxifen‑resistant breast cancer malignant phenotype by proliferation inhibition with bromosulfonamidine amino‑podophyllotoxin

doi: 10.3892/ol.2024.14506

Figure Lengend Snippet: Effect of BSAPPT on apoptosis and cycle-related gene or protein expression in MCF-7, MCF7/TAMR and other cancer cells. (A) mRNA expression levels of genes linked to the cell cycle and apoptosis were measured by qPCR both before and after MCF-7 and MCF7/TAMR cells were treated with 10 µg/ml BSAPPT. (B) Western blotting detection of apoptotic and cycle-related protein expression variations in MCF-7 and MCF7/TAMR cells before and after using 10 µg/ml BSAPPT. Results of qPCR analysis that assessed differences in the level of expression of genes linked to the cell cycle and apoptosis before and after (C) A549 and (D) MDA-MB-231 cells were treated with 10 µg/ml BSAPPT. *P<0.05; **P<0.01; ***P<0.001. BSAPPT, bromosulfonamidine amino-podophyllotoxin; qPCR, quantitative PCR; Bcl-2, B-cell lymphoma 2; Caspase, cysteine aspartic acid-specific protease; PLK, polo like kinase; CCNB1, cyclin B1; TPX2, targeting protein for Xklp2; Bax, Bcl-2 associated X; Cyt-C, cytochrome c; Apaf-1, apoptotic protease activating factor 1.

Article Snippet: Mouse anti-human Caspase-9 antibodies (cat. no. 9508S; 1:1,000) were purchased from Cell Signaling Technology, Inc., rabbit anti-human Bcl-2 (cat. no. BA0412; 1:1,000) and cyclin B1 (CCNB1; cat. no. BA0766; 1:1,000) antibodies were purchased from Wuhan Boster Biological Technology, Ltd., rabbit anti-human polo like kinase (PLK)-1 antibodies (cat. no. 10305-1-AP; 1:1,000) and targeting protein for Xklp2 (TPX2) antibodies (cat. no. 11741-1-AP; 1:2,000) were purchased from Proteintech Group, Inc., and the rabbit anti-human PLK-4 antibodies (cat. no. A9863; 1:1,000) were purchased from ABclonal Biotech Co., Ltd.

Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction